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Cryopreservation and Freeze-Drying Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Willem F. Wolkers

    1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Harriëtte Oldenhof

    1. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1257)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Fundamental Aspects and Freezing Technology

    1. Front Matter

      Pages 1-1
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    2. Principles of Cryopreservation

      • David E. Pegg
      Pages 3-19

    3. Principles of Cryopreservation by Vitrification

      • Gregory M. Fahy, Brian Wowk
      Pages 21-82

    4. Modeling and Optimization of Cryopreservation

      • James D. Benson
      Pages 83-120

    5. The Principles of Freeze-Drying

      • Gerald D. J. Adams, Isobel Cook, Kevin R. Ward
      Pages 121-143

  3. Methods to Study Freezing and Drying Processes

    1. Front Matter

      Pages 145-145
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    2. Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells

      • Willem F. Wolkers, Harriëtte Oldenhof
      Pages 147-161

    3. Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations

      • Wendell Q. Sun
      Pages 163-179

    4. Measurement of Intracellular Ice Formation Kinetics by High-Speed Video Cryomicroscopy

      • Jens O. M. Karlsson
      Pages 181-227

    5. Laser Scanning Microscopy in Cryobiology

      • Frank Stracke, Asger Kreiner-Møller, Heiko Zimmermann
      Pages 229-241

    6. Low-Temperature Electron Microscopy: Techniques and Protocols

      • Roland A. Fleck
      Pages 243-274

  4. Cryopreservation Protocols

    1. Front Matter

      Pages 275-275
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    2. Cryopreservation of Semen from Domestic Livestock

      • Harald Sieme, Harriëtte Oldenhof
      Pages 277-287

    3. Cryopreservation of Mammalian Oocytes

      • Victoria Keros, Barry J. Fuller
      Pages 289-304

    4. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts

      • Juergen Liebermann
      Pages 305-319

    5. Efficient Cryopreservation of Human Pluripotent Stem Cells by Surface-Based Vitrification

      • Julia C. Neubauer, Axel F. Beier, Niels Geijsen, Heiko Zimmermann
      Pages 321-328

    6. Cryopreservation of Greenshell™ Mussel (Perna canaliculus) Sperm

      • Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. McGowan, H. Robin Tervit
      Pages 329-336

    7. Membrane Modification Strategies for Cryopreservation

      • Phillip H. Purdy, James K. Graham
      Pages 337-342

    8. Sperm Cleanup and Centrifugation Processing for Cryopreservation

      • Harald Sieme, Harriëtte Oldenhof
      Pages 343-352
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Keywords

About this book

This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.

Editors and Affiliations

  • Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  • Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

Bibliographic Information

  • Book Title: Cryopreservation and Freeze-Drying Protocols

  • Editors: Willem F. Wolkers, Harriëtte Oldenhof

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2193-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2192-8Published: 27 November 2014

  • Softcover ISBN: 978-1-4939-4912-0Published: 22 September 2016

  • eBook ISBN: 978-1-4939-2193-5Published: 26 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 509

  • Number of Illustrations: 55 b/w illustrations, 35 illustrations in colour

  • Topics: Biotechnology, Biomaterials

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