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Protein Cages

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Brendan P. Orner

    1. Department of Chemistry, King's College London, London, United Kingdom

    View editor publications

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  • Includes cutting-edge methods and protocols to explore protein cages
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1252)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Encapsulation of Nanoparticles in Virus Protein Shells

    • Irina B. Tsvetkova, Bogdan G. Dragnea
    Pages 1-15

  3. Use of Protein Cages as a Template for Confined Synthesis of Inorganic and Organic Nanoparticles

    • Masaki Uchida, Shefah Qazi, Ethan Edwards, Trevor Douglas
    Pages 17-25

  4. Ferritin Encapsulation and Templated Synthesis of Inorganic Nanoparticles

    • Katherine W. Pulsipher, Ivan J. Dmochowski
    Pages 27-37

  5. Determining the Relaxivity Values of Protein Cage-Templated Nanoparticles Using Magnetic Resonance Imaging

    • Barindra Sana, Sierin Lim
    Pages 39-50

  6. Computationally Assisted Engineering of Protein Cages

    • Maziar S. Ardejani, Brendan P. Orner
    Pages 51-59

  7. Recombinant Expression and Purification of “Virus-like” Bacterial Encapsulin Protein Cages

    • W. Frederik Rurup, Jeroen J. L. M. Cornelissen, Melissa S. T. Koay
    Pages 61-67

  8. Production of Bacterial Microcompartments

    • Jonathan K. Lassila
    Pages 69-77

  9. Detection of Protein Cage Assembly with Bisarsenic Fluorescent Probes

    • Thomas A. Cornell, Brendan P. Orner
    Pages 79-89

  10. Determining the Role of Metal Binding in Protein Cage Assembly

    • Anne Grove, Ambuj K. Kushwaha, Khoa H. Nguyen
    Pages 91-100

  11. Differential Scanning Calorimetry to Quantify the Stability of Protein Cages

    • Yu Zhang, Maziar S. Ardejani
    Pages 101-113

  12. Material Properties of Viral Nanocages Explored by Atomic Force Microscopy

    • Mariska G. M. van Rosmalen, Wouter H. Roos, Gijs J. L. Wuite
    Pages 115-137

  13. Computational Mechanics of Viral Capsids

    • Melissa M. Gibbons, Luigi E. Perotti, William S. Klug
    Pages 139-188

  14. Back Matter

    Pages 189-190
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Keywords

About this book

This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents. Protein cages are currently inspiring diverse scientific disciplines and are therefore at the crossroads of extremely widely-scoped research, which is reflected in the detailed chapters of Protein Cages: Methods and Protocols. From nanomaterials studies and iron particles to computational strategies and Atomic Force Microscopy, the chapters herein collectively provide an introduction to the rich world of protein cage research and specific techniques to understand and exploit this fascinating class of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters begin with an introduction to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Protein Cages: Methods and Protocols will help to inspire and further propel the current multi-disciplinary enthusiasm in studying and discovering new applications for protein cages.

Editors and Affiliations

  • Department of Chemistry, King's College London, London, United Kingdom

    Brendan P. Orner

Bibliographic Information

  • Book Title: Protein Cages

  • Book Subtitle: Methods and Protocols

  • Editors: Brendan P. Orner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2131-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2130-0Published: 31 October 2014

  • Softcover ISBN: 978-1-4939-4670-9Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2131-7Published: 30 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 190

  • Number of Illustrations: 28 b/w illustrations, 32 illustrations in colour

  • Topics: Protein Science, Biomaterials

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