Protocols in In Vitro Hepatocyte Research
Editors: Vinken, Mathieu, Rogiers, Vera (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.
- Table of contents (30 chapters)
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Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments
Pages 3-12
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Cryopreservation of Hepatocytes
Pages 13-26
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Culture Conditions Promoting Hepatocyte Proliferation and Cell Cycle Synchronization
Pages 27-51
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Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
Pages 53-76
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Culture and Functional Characterization of Human Hepatoma HepG2 Cells
Pages 77-93
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Table of contents (30 chapters)
- Download Preface 1 PDF (116.8 KB)
- Download Sample pages 1 PDF (945.3 KB)
- Download Table of contents PDF (103.1 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Protocols in In Vitro Hepatocyte Research
- Editors
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- Mathieu Vinken
- Vera Rogiers
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1250
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2074-7
- DOI
- 10.1007/978-1-4939-2074-7
- Hardcover ISBN
- 978-1-4939-2073-0
- Softcover ISBN
- 978-1-4939-4932-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVI, 406
- Number of Illustrations
- 46 b/w illustrations, 26 illustrations in colour
- Topics
