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Stem Cell Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Ivan N. Rich

    1. HemoGenix, Inc., Colorado Springs, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1235)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Short Primer in Stem Cell Biology

    • Ivan N. Rich
    Pages 1-6

  3. Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal, and Expansion Potential

    • Ivan N. Rich
    Pages 7-17

  4. Measuring the Aging Process in Stem Cells

    • Yi Liu, Gary Van Zant, Ying Liang
    Pages 19-32

  5. Measuring the Potency of a Stem Cell Therapeutic

    • Holli Harper, Ivan N. Rich
    Pages 33-48

  6. Culturing Protocols for Human Multipotent Adult Stem Cells

    • Bart Vaes, Sara Walbers, Kristel Gijbels, David Craeye, Robert Deans, Jef Pinxteren et al.
    Pages 49-58

  7. Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial Cells

    • Peter A. G. McCourt, Ana Oteiza, Benjamin Cao, Susan K. Nilsson
    Pages 59-71

  8. Analysis of Circadian Rhythms in Embryonic Stem Cells

    • Jiffin K. Paulose, Edmund B. Rucker III, Vincent M. Cassone
    Pages 73-79

  9. Measuring Stem Cell Circadian Rhythm

    • William Hrushesky, Ivan N. Rich
    Pages 81-95

  10. Cryopreservation of Human Pluripotent Stem Cells: A General Protocol

    • Takamichi Miyazaki, Hirofumi Suemori
    Pages 97-104

  11. Biological Differences Between Native and Cultured Mesenchymal Stem Cells: Implications for Therapies

    • Elena Jones, Richard Schäfer
    Pages 105-120

  12. The Use of Multiparameter Flow Cytometry and Cell Sorting to Characterize Native Human Bone Marrow Mesenchymal Stem Cells (MSC)

    • Sally Boxall, Elena Jones
    Pages 121-130

  13. High Yield Recovery of Equine Mesenchymal Stem Cells from Umbilical Cord Matrix/Wharton’s Jelly Using a Semi-automated Process

    • Timo Z. Nazari-Shafti, Ivone G. Bruno, Rudy F. Martinez, Michael E. Coleman, Eckhard U. Alt, Scott R. McClure
    Pages 131-146

  14. Isolation and Functional Assessment of Cutaneous Stem Cells

    • Yanne S. Doucet, David M. Owens
    Pages 147-164

  15. Isolation of Adult Stem Cell Populations from the Human Cornea

    • Matthew J. Branch, Wing-Yan Yu, Carl Sheridan, Andrew Hopkinson
    Pages 165-177

  16. Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche

    • Isobel Massie, Marc Dziasko, Alvena Kureshi, Hannah J. Levis, Louise Morgan, Michael Neale et al.
    Pages 179-202

  17. Isolation and Characterization of Stem Cells in the Adult Mammalian Ovary

    • Seema Parte, Hiren Patel, Kalpana Sriraman, Deepa Bhartiya
    Pages 203-229

  18. Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells

    • Jonathan L. McQualter, Ivan Bertoncello
    Pages 231-241

  19. Culture and Characterization of Mammary Cancer Stem Cells in Mammospheres

    • Eleonora Piscitelli, Cinzia Cocola, Frank Rüdiger Thaden, Paride Pelucchi, Brian Gray, Giovanni Bertalot et al.
    Pages 243-262

  20. Isolation and Culture of Primary Glioblastoma Cells from Human Tumor Specimens

    • Sascha Seidel, Boyan K. Garvalov, Till Acker
    Pages 263-275
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Keywords

About this book

This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

Editors and Affiliations

  • HemoGenix, Inc., Colorado Springs, USA

    Ivan N. Rich

Bibliographic Information

  • Book Title: Stem Cell Protocols

  • Editors: Ivan N. Rich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1785-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1784-6Published: 11 November 2014

  • Softcover ISBN: 978-1-4939-5531-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1785-3Published: 11 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 281

  • Number of Illustrations: 9 b/w illustrations, 47 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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