Methods in Molecular Biology

The Nucleus

Editors: Hancock, Ronald (Ed.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-1680-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $119.00
price for USA in USD
Softcover $119.00
price for USA in USD
About this book

This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Cell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals

    Pages 3-14

    Steiner, Florian A. (et al.)

  • Lysis Gradient Centrifugation: A Flexible Method for the Isolation of Nuclei from Primary Cells

    Pages 15-23

    Katholnig, Karl (et al.)

  • Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm

    Pages 25-32

    Hancock, Ronald (et al.)

  • A New Rapid Method for Isolating Nucleoli

    Pages 35-42

    Li, Zhou Fang (et al.)

  • Sequential Recovery of Macromolecular Components of the Nucleolus

    Pages 43-51

    Bai, Baoyan (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-1680-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $119.00
price for USA in USD
Softcover $119.00
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The Nucleus
Editors
  • Ronald Hancock
Series Title
Methods in Molecular Biology
Series Volume
1228
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1680-1
DOI
10.1007/978-1-4939-1680-1
Hardcover ISBN
978-1-4939-1679-5
Softcover ISBN
978-1-4939-4873-4
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 237
Number of Illustrations
19 b/w illustrations, 35 illustrations in colour
Topics