Rhinoviruses
Methods and Protocols
Editors: Jans, David A., Ghildyal, Reena (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.
- Table of contents (14 chapters)
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Classification and Evolution of Human Rhinoviruses
Pages 1-10
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Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
Pages 11-24
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Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples
Pages 25-38
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Molecular Genotyping of Human Rhinovirus by Using PCR and Sanger Sequencing
Pages 39-47
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Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions
Pages 49-61
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Table of contents (14 chapters)
- Download Preface 1 PDF (85.3 KB)
- Download Sample pages 1 PDF (296.3 KB)
- Download Table of contents PDF (92.2 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Rhinoviruses
- Book Subtitle
- Methods and Protocols
- Editors
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- David A. Jans
- Reena Ghildyal
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1221
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-1571-2
- DOI
- 10.1007/978-1-4939-1571-2
- Hardcover ISBN
- 978-1-4939-1570-5
- Softcover ISBN
- 978-1-4939-5533-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 190
- Number of Illustrations
- 20 b/w illustrations, 17 illustrations in colour
- Topics
