Overview
- Expands upon the previous edition with current detailed reviews that cover mast cell neophytes and cognoscenti alike
- Features cutting-edge, readily reproducible protocols
- Includes expert notes on troubleshooting
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1220)
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Table of contents (32 protocols)
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Front Matter
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Ontology, Phylogeny, and Tissue Distribution of Mast Cells
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Front Matter
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Mast Cells in Human Health and Disease
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Front Matter
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Molecular Mechanisms of Mast Cell Function
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Front Matter
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Keywords
- allergic disease
- auto-inflammation
- cancer
- cell surface phenotype
- developmental ontology
- disease
- enumeration of tissue mast cells
- experimental mouse models of disease
- isolation of mature mast cells
- mast cell activation state
- mast cell biology
- mast cell progenitors from mammalian tissues
- mast cell secretory
- mast cells in human health
- methods for the isolation
- phylogenetic profile
- tissue fibrosis
About this book
Mast Cells: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike. With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues, Danio rerio (zebrafish) model in the study of mast cell development and function, the functions of mast cells in human health and disease and methods for the isolation, methods for the investigation of the molecular mechanisms of mast cell activation and their effector functions, assays for the detection and analysis of mast cell secretory and cell surface phenotype and, mast cell activation state, and experimental mouse models of disease that have been deemed useful for the assessment of mast cell functions in the regulation of innate and adaptive immune response in cancer, tissue fibrosis, auto-inflammation and allergicdisease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mast Cells: Methods and Protocols, Second Edition provides a sampler of useful methods and techniques in the further study of mast cell biology.
Reviews
“Cutting-edge, easily reproducible protocols and methods for the isolation of mast cells and investigation of the molecular mechanisms of their activation and functions can help investigators plan and carry out their experiments. … aimed primarily at laboratory researchers but also may be useful for all specialists who are interested in mast cell biology. The book is well written, illustrated, summarizes extensive recommendations of world-renowned experts in the field and offers an up-to-date and in-depth look into mast cell biology.” (Pavel Kolkhir, World Allergy Organization, WAO Medical Book Reviews, worldallergy.org, July, 2016)
Editors and Affiliations
Bibliographic Information
Book Title: Mast Cells
Book Subtitle: Methods and Protocols
Editors: Michael R. Hughes, Kelly M. McNagny
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1568-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1567-5Published: 13 November 2014
Softcover ISBN: 978-1-4939-5170-3Published: 22 September 2016
eBook ISBN: 978-1-4939-1568-2Published: 11 November 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XVII, 540
Number of Illustrations: 18 b/w illustrations, 46 illustrations in colour
Topics: Cell Biology