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  • © 2014

Stem Cells and Tissue Repair

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1210)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
  2. Culturing and Differentiating Mouse Embryonic Stem Cells

    • Axel P. Gross, Chrissa Kioussi
    Pages 1-8
  3. Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration

    • Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jiménez et al.
    Pages 23-35
  4. Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for In Vivo Analysis of the Brain Tumor-Initiating Cell

    • Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora et al.
    Pages 37-49
  5. Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells

    • Qiuyue Liu, Andrzej Swistowski, Xianmin Zeng
    Pages 79-90
  6. Dental Pulp Stem Cell (DPSC) Isolation, Characterization, and Differentiation

    • Federico Ferro, Renza Spelat, Chelsea S. Baheney
    Pages 91-115
  7. Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering

    • Adriana Di Benedetto, Claudia Carbone, Giorgio Mori
    Pages 117-130
  8. Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Three-Dimensional Microscale Culture

    • Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura et al.
    Pages 131-141
  9. The Generation and Maintenance of Rat Induced Pluripotent Stem Cells

    • Tomoyuki Yamaguchi, Sanae Hamanaka, Hiromitsu Nakauchi
    Pages 143-150
  10. Adipose-Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

    • Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok et al.
    Pages 161-181
  11. Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes

    • Marie Schneider, Christina Dieckmann, Katrin Rabe, Jan-Christoph Simon, Vuk Savkovic
    Pages 203-227
  12. Back Matter

    Pages 249-250

About this book

Stem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas. The first approach taps into the latent regenerative capacity of particular tissues, such as muscle, skin, fat, or bone marrow. The second approach induces and grows pluripotent stem cells, drives their differentiation along certain pathways such as germ layers, neural crest, liver, teeth or retina, and cultures organs such as pancreas and heart. While the third approach involves various engraftment techniques for neural tissue. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Stem Cells and Tissue Repair: Methods and Protocols provides state-of the-art methods descriptions and the references therein that will provide a suitable starting point for exploring the vast literature that has already developed for regenerative medicine.

Editors and Affiliations

  • Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, USA

    Chrissa Kioussi

Bibliographic Information

  • Book Title: Stem Cells and Tissue Repair

  • Book Subtitle: Methods and Protocols

  • Editors: Chrissa Kioussi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1435-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1434-0Published: 01 September 2014

  • Softcover ISBN: 978-1-4939-5532-9Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1435-7Published: 30 August 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 250

  • Number of Illustrations: 8 b/w illustrations, 42 illustrations in colour

  • Topics: Cell Biology, Stem Cells

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access