- Includes cutting-edge methods and protocols involving the plant endosome
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.
- Table of contents (20 chapters)
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The Use of FM Dyes to Analyze Plant Endocytosis
Pages 1-11
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Sterol Dynamics During Endocytic Trafficking in Arabidopsis
Pages 13-29
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Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs
Pages 31-44
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Analysis of Fluid-Phase Endocytosis in (Intact) Plant Cells
Pages 45-61
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Immunogold Labeling and Electron Tomography of Plant Endosomes
Pages 63-80
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Endosomes
- Book Subtitle
- Methods and Protocols
- Editors
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- Marisa S. Otegui
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1209
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- Distribution Rights
- Distribution rights for India: Delhi Book Store, New Delhi, India
- eBook ISBN
- 978-1-4939-1420-3
- DOI
- 10.1007/978-1-4939-1420-3
- Hardcover ISBN
- 978-1-4939-1419-7
- Softcover ISBN
- 978-1-4939-4586-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 286
- Number of Illustrations
- 21 b/w illustrations, 30 illustrations in colour
- Topics
