Methods in Molecular Biology

Plant Endosomes

Methods and Protocols

Editors: Otegui, Marisa S. (Ed.)

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  • Includes cutting-edge methods and protocols involving the plant endosome
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Hardcover $169.99
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Softcover $119.00
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About this book

Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • The Use of FM Dyes to Analyze Plant Endocytosis

    Pages 1-11

    Malínská, Kateřina (et al.)

  • Sterol Dynamics During Endocytic Trafficking in Arabidopsis

    Pages 13-29

    Stanislas, Thomas (et al.)

  • Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs

    Pages 31-44

    Ovečka, Miroslav (et al.)

  • Analysis of Fluid-Phase Endocytosis in (Intact) Plant Cells

    Pages 45-61

    Bandmann, Vera (et al.)

  • Immunogold Labeling and Electron Tomography of Plant Endosomes

    Pages 63-80

    Chanoca, Alexandra (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-1420-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-1419-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4586-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Plant Endosomes
Book Subtitle
Methods and Protocols
Editors
  • Marisa S. Otegui
Series Title
Methods in Molecular Biology
Series Volume
1209
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
Distribution Rights
Distribution rights for India: Delhi Book Store, New Delhi, India
eBook ISBN
978-1-4939-1420-3
DOI
10.1007/978-1-4939-1420-3
Hardcover ISBN
978-1-4939-1419-7
Softcover ISBN
978-1-4939-4586-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 286
Number of Illustrations
21 b/w illustrations, 30 illustrations in colour
Topics