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Methods in Molecular Biology

Galectins

Methods and Protocols

Editors: Stowell, Sean R., Cummings, Richard D. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-1396-1
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-1395-4
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  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
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  • ISBN 978-1-4939-5296-0
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About this book

Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function.  Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Evolving Mechanistic Insights into Galectin Functions

    Pages 1-35

    Arthur, Connie M. (et al.)

  • Cloning, Expression, and Purification of Galectins for In Vitro Studies

    Pages 37-49

    Poland, Paul A. (et al.)

  • Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation

    Pages 51-62

    Stowell, Sean R. (et al.)

  • Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC)

    Pages 63-74

    Iwaki, Jun (et al.)

  • Probing Lectin–Mucin Interactions by Isothermal Titration Microcalorimetry

    Pages 75-90

    Dam, Tarun K. (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-1396-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-1395-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-1-4939-5296-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Galectins
Book Subtitle
Methods and Protocols
Editors
  • Sean R. Stowell
  • Richard D. Cummings
Series Title
Methods in Molecular Biology
Series Volume
1207
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1396-1
DOI
10.1007/978-1-4939-1396-1
Hardcover ISBN
978-1-4939-1395-4
Softcover ISBN
978-1-4939-5296-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 488
Number of Illustrations
48 b/w illustrations, 52 illustrations in colour
Topics