Galectins
Methods and Protocols
Editors: Stowell, Sean R., Cummings, Richard D. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.
- Table of contents (30 chapters)
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Evolving Mechanistic Insights into Galectin Functions
Pages 1-35
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Cloning, Expression, and Purification of Galectins for In Vitro Studies
Pages 37-49
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Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation
Pages 51-62
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Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC)
Pages 63-74
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Probing Lectin–Mucin Interactions by Isothermal Titration Microcalorimetry
Pages 75-90
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Table of contents (30 chapters)
- Download Preface 1 PDF (65.9 KB)
- Download Sample pages 1 PDF (389.3 KB)
- Download Table of contents PDF (122 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Galectins
- Book Subtitle
- Methods and Protocols
- Editors
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- Sean R. Stowell
- Richard D. Cummings
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1207
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-1396-1
- DOI
- 10.1007/978-1-4939-1396-1
- Hardcover ISBN
- 978-1-4939-1395-4
- Softcover ISBN
- 978-1-4939-5296-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 488
- Number of Illustrations
- 48 b/w illustrations, 52 illustrations in colour
- Topics
