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Biomimetics and Stem Cells

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Gordana Vunjak-Novakovic

    1. Department of Biomedical Engineering and Department of Medicine, Columbia University, New York, USA

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  2. Kursad Turksen

    1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols on the use of biomimetic systems with stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1202)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Derivation and Network Formation of Vascular Cells from Human Pluripotent Stem Cells

    • Sravanti Kusuma, Bria Macklin, Sharon Gerecht
    Pages 1-9

  3. High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis

    • Ahmad E. Abu-Hakmeh, Leo Q. Wan
    Pages 11-19

  4. Microfluidic Device to Culture 3D In Vitro Human Capillary Networks

    • Monica L. Moya, Luis F. Alonzo, Steven C. George
    Pages 21-27

  5. Multifunction Co-culture Model for Evaluating Cell–Cell Interactions

    • Danielle R. Bogdanowicz, Helen H. Lu
    Pages 29-36

  6. Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography

    • Camila Londono, John Soleas, Petra B. Lücker, Suthamathy Sathananthan, J. Stewart Aitchison, Alison P. McGuigan
    Pages 37-55

  7. Bioreactor Cultivation of Anatomically Shaped Human Bone Grafts

    • Joshua P. Temple, Keith Yeager, Sarindr Bhumiratana, Gordana Vunjak-Novakovic, Warren L. Grayson
    Pages 57-78

  8. Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells

    • KangAe Lee, Celeste M. Nelson
    Pages 79-94

  9. Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules

    • Anthony Conway, Dawn P. Spelke, David V. Schaffer
    Pages 95-102

  10. An Assay to Quantify Chemotactic Properties of Degradation Products from Extracellular Matrix

    • Brian M. Sicari, Li Zhang, Ricardo Londono, Stephen F. Badylak
    Pages 103-110

  11. Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment

    • Ana M. Martins, Rui L. Reis
    Pages 111-119

  12. Fabrication of Biofunctionalized, Cell-Laden Macroporous 3D PEG Hydrogels as Bone Marrow Analogs for the Cultivation of Human Hematopoietic Stem and Progenitor Cells

    • Lisa Rödling, Annamarija Raic, Cornelia Lee-Thedieck
    Pages 121-130

  13. Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation

    • Busra Mammadov, Mustafa O. Guler, Ayse B. Tekinay
    Pages 131-148

  14. The Delivery and Evaluation of RNAi Therapeutics for Heterotopic Ossification Pathologies

    • Arun R. Shrivats, Jeffrey O. Hollinger
    Pages 149-160

  15. Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation

    • ZuFu Lu, Seyed-Iman Roohani-Esfahani, Hala Zreiqat
    Pages 161-171

  16. Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in Perfusion Bioreactors

    • Giuseppe Maria de Peppo, Gordana Vunjak-Novakovic, Darja Marolt
    Pages 173-184

  17. Back Matter

    Pages 185-187
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Keywords

About this book

Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to ‘‘instruct’’ stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and essential, Biomimetics and Stem Cells: Methods and Protocols focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward.

Editors and Affiliations

  • Department of Biomedical Engineering and Department of Medicine, Columbia University, New York, USA

    Gordana Vunjak-Novakovic

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Biomimetics and Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Gordana Vunjak-Novakovic, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1332-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1331-2Published: 02 July 2014

  • Softcover ISBN: 978-1-4939-4431-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1332-9Published: 30 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 187

  • Number of Illustrations: 23 b/w illustrations, 31 illustrations in colour

  • Topics: Cell Biology, Stem Cells, Regenerative Medicine/Tissue Engineering

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