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Epidermal Cells

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Kursad Turksen

    1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    View editor publications

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  • Includes cutting-edge methods and protocols on epidermal cell research
  • Fully updated from the previous editions
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1195)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage

    • Igor Kogut, Dennis R. Roop, Ganna Bilousova
    Pages 1-12

  3. Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells

    • Fahad K. Kidwai, Tong Cao, Kai Lu
    Pages 13-22

  4. Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds

    • Dongxia Ye, Antonio Peramo
    Pages 23-32

  5. Growth and Differentiation of HaCaT Keratinocytes

    • Van G. Wilson
    Pages 33-41

  6. Transgene Delivery to Cultured Keratinocytes via Replication-Deficient Adenovirus Vectors

    • Vincent P. Ramirez, Brian J. Aneskievich
    Pages 43-48

  7. Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq

    • Jason M. Rizzo, Satrajit Sinha
    Pages 49-59

  8. Analysis and Meta-analysis of Transcriptional Profiling in Human Epidermis

    • Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, Miroslav Blumenberg
    Pages 61-97

  9. Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline

    • Raphaela Rid, Harald Hundsberger, Kamil Önder
    Pages 99-109

  10. Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity

    • Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, Wendy B. Bollag
    Pages 111-131

  11. Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes

    • Kathleen P. McGuinn, Mỹ G. Mahoney
    Pages 133-144

  12. MMP-2, -9 and TIMP-1, -2 Assays in Keratinocyte Cultures

    • Takashi Kobayashi
    Pages 145-155

  13. Reactive Oxygen Species (ROS) Protection via Cysteine Oxidation in the Epidermal Cornified Cell Envelope

    • Wilbert P. Vermeij, Claude Backendorf
    Pages 157-169

  14. Modified Methods for Growing 3-D Skin Equivalents: An Update

    • Rebecca Lamb, Carrie A. Ambler
    Pages 171-182

  15. A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro

    • Yoji Okugawa, Yohei Hirai
    Pages 183-190

  16. Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter

    • Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert de Rouvroit, Yves Poumay
    Pages 191-201

  17. Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications

    • John J. Wille, Jeremy J. Burdge, Jong Y. Park
    Pages 203-218

  18. Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection

    • Laura M. Griffin, Louis Cicchini, Tao Xu, Dohun Pyeon
    Pages 219-238

  19. Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle

    • Christine Chevalier, Jean-François Nicolas, Anne-Cécile Petit
    Pages 239-245

  20. Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies

    • Inês Sequeira, Emilie Legué, Suzanne Capgras, Jean-François Nicolas
    Pages 247-258
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Keywords

About this book

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Epidermal Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1224-7

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1223-0Published: 14 July 2014

  • Softcover ISBN: 978-1-4939-5578-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1224-7Published: 11 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 296

  • Number of Illustrations: 116 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology, Stem Cells, Cell Culture

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