- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.
- Table of contents (16 chapters)
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Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8+ T Cells from Blood and Tumor Tissue
Pages 1-11
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In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes
Pages 13-20
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Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry
Pages 21-47
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Intracellular Signaling of CTLs
Pages 49-63
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Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
Pages 65-74
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Cytotoxic T-Cells
- Book Subtitle
- Methods and Protocols
- Editors
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- Elena Ranieri
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1186
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-1158-5
- DOI
- 10.1007/978-1-4939-1158-5
- Hardcover ISBN
- 978-1-4939-1157-8
- Softcover ISBN
- 978-1-4939-4686-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 301
- Number of Illustrations
- 6 b/w illustrations, 26 illustrations in colour
- Topics
