Methods in Molecular Biology

Patch-Clamp Methods and Protocols

Editors: Martina, Marzia, Taverna, Stefano (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Patch-Cla

mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Reviews

“This is a very good up to date ‘how to’ manual with dozens of unique protocols regarding single channel, whole cell, and network patch recordings. … This atlas and textbook is a good choice for ‘patchers’, neurohysiologists, biophysicists, students, fellows and ‘cell’ molecular physiologists.” (Joseph J. Grenier, Amazon.com, September, 2015)


Table of contents (23 chapters)

Table of contents (23 chapters)
  • History of Electrophysiology and the Patch Clamp

    Verkhratsky, Alexei (et al.)

    Pages 1-19

  • Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications

    Glasgow, Nathan G. (et al.)

    Pages 23-41

  • Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-Methyl-d-aspartate (NMDA) Receptor Open Channel Blockers

    Parsons, Chris G. (et al.)

    Pages 43-63

  • State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening

    Stoelzle-Feix, Sonja

    Pages 65-80

  • Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch® 96 Using Instant Frozen Cells

    Polonchuk, Liudmila

    Pages 81-92

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-1096-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-1095-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-5414-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Patch-Clamp Methods and Protocols
Editors
  • Marzia Martina
  • Stefano Taverna
Series Title
Methods in Molecular Biology
Series Volume
1183
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1096-0
DOI
10.1007/978-1-4939-1096-0
Hardcover ISBN
978-1-4939-1095-3
Softcover ISBN
978-1-4939-5414-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 403
Number of Illustrations
64 b/w illustrations, 41 illustrations in colour
Topics