Brain Energy Metabolism
Editors: Hirrlinger, Johannes, Waagepetersen, Helle S. (Eds.)
Free Preview- Includes techniques to investigate brain energy metabolism in differing levels of cell and tissue complexity
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab.
Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.
- Table of contents (14 chapters)
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Determination of CO2 Production in Subcellular Preparations Like Synaptosomes and Isolated Mitochondria Using 14C-Labeled Substrates and Radioactive CO2 Measurements
Pages 1-24
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Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes
Pages 25-43
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Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells
Pages 45-72
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Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS)
Pages 73-105
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Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro
Pages 107-144
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Table of contents (14 chapters)
- Download Preface 1 PDF (92.7 KB)
- Download Sample pages 1 PDF (839.5 KB)
- Download Table of contents PDF (110.9 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Brain Energy Metabolism
- Editors
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- Johannes Hirrlinger
- Helle S. Waagepetersen
- Series Title
- Neuromethods
- Series Volume
- 90
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-1059-5
- DOI
- 10.1007/978-1-4939-1059-5
- Hardcover ISBN
- 978-1-4939-1058-8
- Softcover ISBN
- 978-1-4939-4466-8
- Series ISSN
- 0893-2336
- Edition Number
- 1
- Number of Pages
- XIV, 368
- Number of Illustrations
- 45 b/w illustrations, 52 illustrations in colour
- Topics
