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Methods in Molecular Biology

Protein Affinity Tags

Methods and Protocols

Editors: Giannone, Richard J., Dykstra, Andrew B. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-1034-2
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Hardcover $199.99
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About this book

Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • 1D4: A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins

    Pages 1-15

    Molday, Laurie L. (et al.)

  • Affinity Purification of Heme-Tagged Proteins

    Pages 17-33

    Asher, Wesley B. (et al.)

  • Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag

    Pages 35-45

    Wang, Dongmei (et al.)

  • Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag

    Pages 47-58

    Coolbaugh, Michael J. (et al.)

  • Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag

    Pages 59-70

    Shen, Aimee

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4939-1034-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $129.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Protein Affinity Tags
Book Subtitle
Methods and Protocols
Editors
  • Richard J. Giannone
  • Andrew B. Dykstra
Series Title
Methods in Molecular Biology
Series Volume
1177
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1034-2
DOI
10.1007/978-1-4939-1034-2
Hardcover ISBN
978-1-4939-1033-5
Softcover ISBN
978-1-4939-4139-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 213
Number of Illustrations
15 b/w illustrations, 31 illustrations in colour
Topics