Protein Affinity Tags
Methods and Protocols
Editors: Giannone, Richard J., Dykstra, Andrew B. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein’s function and structure. Today’s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to “fish out” a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information – information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today’s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest.
- Table of contents (15 chapters)
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1D4: A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins
Pages 1-15
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Affinity Purification of Heme-Tagged Proteins
Pages 17-33
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Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag
Pages 35-45
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Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag
Pages 47-58
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Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag
Pages 59-70
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Table of contents (15 chapters)
- Download Preface 1 PDF (35.3 KB)
- Download Sample pages 1 PDF (350.1 KB)
- Download Table of contents PDF (47.6 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Protein Affinity Tags
- Book Subtitle
- Methods and Protocols
- Editors
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- Richard J. Giannone
- Andrew B. Dykstra
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1177
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-1034-2
- DOI
- 10.1007/978-1-4939-1034-2
- Hardcover ISBN
- 978-1-4939-1033-5
- Softcover ISBN
- 978-1-4939-4139-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 213
- Number of Illustrations
- 15 b/w illustrations, 31 illustrations in colour
- Topics
