Methods in Molecular Biology

Exocytosis and Endocytosis

Editors: Ivanov, Andrei I. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Hardcover $219.99
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About this book

Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets

    Pages 3-18

    Ivanov, Andrei I.

  • Systematic Analysis of Endocytosis by Cellular Perturbations

    Pages 19-46

    Kühling, Lena (et al.)

  • Real-Time Detection of SNARE Complex Assembly with FRET Using the Tetracysteine System

    Pages 49-55

    Varlamov, Oleg

  • Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides

    Pages 57-71

    Xu, Guoqiang (et al.)

  • Secretion of Circular Proteins Using Sortase

    Pages 73-83

    Strijbis, Karin (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-0944-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-0943-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-4694-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Exocytosis and Endocytosis
Editors
  • Andrei I. Ivanov
Series Title
Methods in Molecular Biology
Series Volume
1174
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0944-5
DOI
10.1007/978-1-4939-0944-5
Hardcover ISBN
978-1-4939-0943-8
Softcover ISBN
978-1-4939-4694-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 435
Number of Illustrations
38 b/w illustrations, 55 illustrations in colour
Topics