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Methods in Molecular Biology

Animal Endo-SiRNAs

Methods and Protocols

Editors: Werner, Andreas (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs. These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Targeted Small Noncoding RNA-Directed Gene Activation in Human Cells

    Pages 1-10

    Damski, Caio (et al.)

  • Isolation of Chromatoid Bodies from Mouse Testis as a Rich Source of Short RNAs

    Pages 11-25

    Meikar, Oliver (et al.)

  • Generation of Endo-siRNAs in Xenopus laevis Oocytes

    Pages 27-32

    Alnumeir, Sammer (et al.)

  • Analysis of Endo-siRNAs in Drosophila

    Pages 33-49

    Elmer, Katharina (et al.)

  • Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

    Pages 51-58

    Lim, Do-Hwan (et al.)

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4939-0931-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-0930-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA in USD
  • ISBN 978-1-4939-4160-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Animal Endo-SiRNAs
Book Subtitle
Methods and Protocols
Editors
  • Andreas Werner
Series Title
Methods in Molecular Biology
Series Volume
1173
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0931-5
DOI
10.1007/978-1-4939-0931-5
Hardcover ISBN
978-1-4939-0930-8
Softcover ISBN
978-1-4939-4160-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 182
Number of Illustrations
12 b/w illustrations, 24 illustrations in colour
Topics

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