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Plant Cold Acclimation

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Dirk K. Hincha

    1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ellen Zuther

    1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1166)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction: Plant Cold Acclimation and Freezing Tolerance

    • Dirk K. Hincha, Ellen Zuther
    Pages 1-6

  3. Measuring Freezing Tolerance: Survival and Regrowth Assays

    • Daniel Z. Skinner, Kimberly Garland-Campbell
    Pages 7-13

  4. Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays

    • Anja Thalhammer, Dirk K. Hincha, Ellen Zuther
    Pages 15-24

  5. Conducting Field Trials for Frost Tolerance Breeding in Cereals

    • Luigi Cattivelli
    Pages 25-33

  6. A Whole-Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance

    • Annick Bertrand, Yves Castonguay, Josée Bourassa
    Pages 35-41

  7. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

    • Evelyne Téoulé, Carine Géry
    Pages 43-64

  8. Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions

    • Andrey V. Malyshev, Hugh A. L. Henry, Juergen Kreyling
    Pages 65-78

  9. Identification of Arabidopsis Mutants with Altered Freezing Tolerance

    • Carlos Perea-Resa, Julio Salinas
    Pages 79-89

  10. Infrared Thermal Analysis of Plant Freezing Processes

    • Gilbert Neuner, Edith Kuprian
    Pages 91-98

  11. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

    • Seizo Fujikawa, Keita Endoh
    Pages 99-116

  12. Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images

    • David P. Livingston III, Tan D. Tuong
    Pages 117-137

  13. Proteomic Approaches to Identify Cold-Regulated Soluble Proteins

    • Stefanie Döll, Rico Lippmann, Hans-Peter Mock
    Pages 139-158

  14. Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

    • Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, Matsuo Uemura
    Pages 159-170

  15. Profiling Methods to Identify Cold-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

    • Frederik Dethloff, Alexander Erban, Isabel Orf, Jessica Alpers, Ines Fehrle, Olga Beine-Golovchuk et al.
    Pages 171-197

  16. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

    • Hieu Sy Vu, Sunitha Shiva, Aaron Smalter Hall, Ruth Welti
    Pages 199-215

  17. Quantification of Superoxide and Hydrogen Peroxide in Leaves

    • Ilona Juszczak, Margarete Baier
    Pages 217-224

  18. Estimating Ice Encasement Tolerance of Herbage Plants

    • Bjarni E. Gudleifsson, Brynhildur Bjarnadottir
    Pages 225-240

  19. Characterization of Ice Binding Proteins from Sea Ice Algae

    • Maddalena Bayer-Giraldi, EonSeon Jin, Peter W. Wilson
    Pages 241-253

  20. Isolation and Characterization of Ice-Binding Proteins from Higher Plants

    • Adam J. Middleton, Barbara Vanderbeld, Melissa Bredow, Heather Tomalty, Peter L. Davies, Virginia K. Walker
    Pages 255-277
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Keywords

About this book

Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.

Editors and Affiliations

  • Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha, Ellen Zuther

Bibliographic Information

  • Book Title: Plant Cold Acclimation

  • Book Subtitle: Methods and Protocols

  • Editors: Dirk K. Hincha, Ellen Zuther

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0844-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0843-1Published: 23 May 2014

  • Softcover ISBN: 978-1-4939-4615-0Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0844-8Published: 22 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 26 b/w illustrations, 49 illustrations in colour

  • Topics: Plant Sciences, Agriculture

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