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Methods in Molecular Biology

Shotgun Proteomics

Methods and Protocols

Editors: Martins-de-Souza, Daniel (Ed.)

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  • Includes cutting-edge methods and protocols involving a wide array of proteome studies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-0685-7
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-0684-0
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
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  • ISBN 978-1-4939-4695-2
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  • Usually dispatched within 3 to 5 business days.
About this book

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.

Table of contents (31 chapters)

Table of contents (31 chapters)
  • Survey of Shotgun Proteomics

    Pages 3-23

    Nogueira, Fabio Cesar Sousa (et al.)

  • LC-MALDI-TOF/TOF for Shotgun Proteomics

    Pages 27-38

    Fernández-Puente, Patricia (et al.)

  • Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry

    Pages 39-51

    Lam, Maggie P. Y. (et al.)

  • GeLC-MS/MS Analysis of Complex Protein Mixtures

    Pages 53-66

    Dzieciatkowska, Monika (et al.)

  • IPG Strip-Based Peptide Fractionation for Shotgun Proteomics

    Pages 67-77

    Eravci, Murat (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-0685-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-0684-0
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4695-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Shotgun Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Daniel Martins-de-Souza
Series Title
Methods in Molecular Biology
Series Volume
1156
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0685-7
DOI
10.1007/978-1-4939-0685-7
Hardcover ISBN
978-1-4939-0684-0
Softcover ISBN
978-1-4939-4695-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 492
Number of Illustrations
33 b/w illustrations, 48 illustrations in colour
Topics