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Membrane Proteins Production for Structural Analysis

  • Book
  • © 2014

Overview

Editors:
  1. Isabelle Mus-Veteau

    1. UMR-CNRS 7275, Institute of Molecular and Cellular Pharmacology, Valbonne, France

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  • Updates the latest development in production, stabilization and structural analysis techniques of membrane proteins

  • Explores the structural characterization of membrane proteins

  • Follows the expression, migration, modifications and recycling of a membrane protein

  • Includes supplementary material: sn.pub/extras

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Table of contents (15 chapters)

  1. Front Matter

    Pages i-xxiii
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  2. Membrane Protein Production for Structural Analysis

    • Isabelle Mus-Veteau, Pascal Demange, Francesca Zito
    Pages 1-44

  3. Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies

    • Davide Proverbio, Erik Henrich, Erika Orbán, Volker Dötsch, Frank Bernhard
    Pages 45-70

  4. Bacterial Expression and Stabilization of GPCRs

    • Jean-Louis Banères
    Pages 71-86

  5. Membrane Protein Production in Escherichia coli: Overview and Protocols

    • Georges Hattab, Annabelle Y. T. Suisse, Oana Ilioaia, Marina Casiraghi, Manuela Dezi, Xavier L. Warnet et al.
    Pages 87-106

  6. Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

    • Sana Bakari, François André, Daphné Seigneurin-Berny, Marcel Delaforge, Norbert Rolland, Annie Frelet-Barrand
    Pages 107-132

  7. Overexpression of Membrane Proteins in Saccharomyces cerevisiae for Structural and Functional Studies: A Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p

    • Cédric Montigny, Hassina Azouaoui, Aurore Jacquot, Marc le Maire, Christine Jaxel, Philippe Champeil et al.
    Pages 133-171

  8. Amphipols: A General Introduction and Some Protocols

    • Manuela Zoonens, Francesca Zito, Karen L. Martinez, Jean-Luc Popot
    Pages 173-203

  9. New Amphiphiles to Handle Membrane Proteins: “Ménage à Trois” Between Chemistry, Physical Chemistry, and Biochemistry

    • Grégory Durand, Maher Abla, Christine Ebel, Cécile Breyton
    Pages 205-251

  10. Building Model Membranes with Lipids and Proteins: Dangers and Challenges

    • James N. Sturgis
    Pages 253-266

  11. Analytical Ultracentrifugation and Size-Exclusion Chromatography Coupled with Light Scattering for the Characterization of Membrane Proteins in Solution

    • Aline Le Roy, Cécile Breyton, Christine Ebel
    Pages 267-287

  12. Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins

    • Andrii Ishchenko, Enrique Abola, Vadim Cherezov
    Pages 289-314

  13. Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR

    • Laurent J. Catoire, Xavier L. Warnet, Dror E. Warschawski
    Pages 315-345

  14. Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins

    • Jérôme Hénin, Marc Baaden, Antoine Taly
    Pages 347-392

  15. Structural Studies of TSPO, a Mitochondrial Membrane Protein

    • Jean-Jacques Lacapere, Soria Iatmanen-Harbi, Lucile Senicourt, Olivier Lequin, Piotr Tekely, Rudra N. Purusottam et al.
    Pages 393-421

  16. Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins

    • Jérôme Hénin, Marc Baaden, Antoine Taly
    Pages E1-E1

  17. Back Matter

    Pages 423-425
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Keywords

About this book

This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.

Editors and Affiliations

  • UMR-CNRS 7275, Institute of Molecular and Cellular Pharmacology, Valbonne, France

    Isabelle Mus-Veteau

About the editor

Dr. Isabelle Mus-Veteau is a biochemist and biophysicist specialist in membrane protein characterization. She obtained her PhD in microbiology and cell biology at the University of Marseille in France. She has held a French National Centre for Scientific Research (CNRS) tenure position at the Institute of Molecular and Cellular Pharmacology (IPMC, Sophia Anti polis near Nice, France), where she supervises projects on the characterization of the Hedgehog receptor Patched. She was a member of the CNRS tenure position recruitment committee and is currently a member of the executive committees of the French Biophysical Society and of the Membrane Group Society. She organized two international summer schools on membrane protein production for structural analysis and two international congresses on membrane biophysics.

Bibliographic Information

  • Book Title: Membrane Proteins Production for Structural Analysis

  • Editors: Isabelle Mus-Veteau

  • DOI: https://doi.org/10.1007/978-1-4939-0662-8

  • Publisher: Springer New York, NY

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0661-1Published: 21 June 2014

  • Softcover ISBN: 978-1-4939-4806-2Published: 03 September 2016

  • eBook ISBN: 978-1-4939-0662-8Published: 20 June 2014

  • Edition Number: 1

  • Number of Pages: XXIII, 425

  • Number of Illustrations: 36 b/w illustrations, 72 illustrations in colour

  • Topics: Cell Biology, Membrane Biology, Protein Science

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