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Flavins and Flavoproteins

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Stefan Weber

    1. Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Erik Schleicher

    1. Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1146)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
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  2. Part I

    1. Front Matter

      Pages 1-1
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    2. Structure and General Properties of Flavins

      • Ana Maria Edwards
      Pages 3-13

    3. Recent Advances in Riboflavin Biosynthesis

      • Ilka Haase, Tobias Gräwert, Boris Illarionov, Adelbert Bacher, Markus Fischer
      Pages 15-40

    4. Natural Riboflavin Analogs

      • Danielle Biscaro Pedrolli, Frank Jankowitsch, Julia Schwarz, Simone Langer, Shinobu Nakanishi, Matthias Mack
      Pages 41-63

    5. A Roadmap to the Isotopolog Space of Flavocoenzymes

      • Adelbert Bacher, Boris Illarionov, Wolfgang Eisenreich, Markus Fischer
      Pages 65-78

    6. Electron Transferases

      • Patricia Ferreira, Marta Martínez-Júlvez, Milagros Medina
      Pages 79-94

    7. Aldonolactone Oxidoreductases

      • Nicole G. H. Leferink, Willem J. H. van Berkel
      Pages 95-111

    8. Flavins and Flavoproteins: Applications in Medicine

      • Esther Jortzik, Lihui Wang, Jipeng Ma, Katja Becker
      Pages 113-157

  3. Part II

    1. Front Matter

      Pages 159-159
      Download chapter PDF

    2. Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms

      • Christopher R. Pudney, Sam Hay, Nigel S. Scrutton
      Pages 161-175

    3. On the In Vivo Redox State of Flavin-Containing Photosensory Receptor Proteins

      • Aleksandra Bury, Klaas J. Hellingwerf
      Pages 177-190

    4. Computational Spectroscopy, Dynamics, and Photochemistry of Photosensory Flavoproteins

      • Tatiana Domratcheva, Anikó Udvarhelyi, Abdul Rehaman Moughal Shahi
      Pages 191-228

    5. NMR Spectroscopy on Flavins and Flavoproteins

      • Franz Müller
      Pages 229-306

    6. Solid-State NMR of Flavins and Flavoproteins

      • Anne-Frances Miller
      Pages 307-340

    7. EPR on Flavoproteins

      • Richard Brosi, Robert Bittl, Christopher Engelhard
      Pages 341-360

    8. FTIR Spectroscopy of Flavin-Binding Photoreceptors

      • Daichi Yamada, Hideki Kandori
      Pages 361-376

    9. Resonance Raman Spectroscopy

      • Jiang Li, Teizo Kitagawa
      Pages 377-400

    10. Photoactivation Mechanisms of Flavin-Binding Photoreceptors Revealed Through Ultrafast Spectroscopy and Global Analysis Methods

      • Tilo Mathes, Ivo H. M. van Stokkum, John T. M. Kennis
      Pages 401-442

    11. A “How-To” Guide to the Stark Spectroscopy of Flavins and Flavoproteins

      • Raymond F. Pauszek, Robert J. Stanley
      Pages 443-466
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Keywords

About this book

In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.

Editors and Affiliations

  • Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Germany

    Stefan Weber, Erik Schleicher

Bibliographic Information

  • Book Title: Flavins and Flavoproteins

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan Weber, Erik Schleicher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0452-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0451-8Published: 25 April 2014

  • Softcover ISBN: 978-1-4939-4170-4Published: 03 September 2016

  • eBook ISBN: 978-1-4939-0452-5Published: 24 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 468

  • Number of Illustrations: 66 b/w illustrations, 70 illustrations in colour

  • Topics: Protein Science

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