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  • © 2014

Crop Breeding

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1145)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Defining a Genetic Ideotype for Crop Improvement

    • Richard M. Trethowan
    Pages 1-20
  3. Genotyping by High-Resolution Melting Analysis

    • Elise J. Tucker, Bao Lam Huynh
    Pages 59-66
  4. SNP Genotyping: The KASP Assay

    • Chunlin He, John Holme, Jeffrey Anthony
    Pages 75-86
  5. Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding

    • Sergío Perez-Elizalde, Jesús J. Cerón-Rojas, José Crossa, Delphine Fleury, Gregorio Alvarado
    Pages 87-96
  6. OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs

    • Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset et al.
    Pages 97-116
  7. Genomic Selection in Plant Breeding

    • Mark A. Newell, Jean-Luc Jannink
    Pages 117-130
  8. Simulated Breeding with QU-GENE Graphical User Interface

    • Adrian Hathorn, Scott Chapman, Mark Dieters
    Pages 131-142
  9. TILLING for Plant Breeding

    • Peter Sharp, Chongmei Dong
    Pages 155-165
  10. In vitro Culture for Doubled Haploids: Tools for Molecular Breeding

    • Sue Broughton, Parminder K. Sidhu, Philip A. Davies
    Pages 167-189
  11. Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin

    • Ainur Ismagul, Gulnur Iskakova, John C. Harris, Serik Eliby
    Pages 191-202
  12. Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)

    • Ainur Ismagul, Iryna Mazonka, Corinne Callegari, Serik Eliby
    Pages 203-211
  13. Back Matter

    Pages 253-255

About this book

The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow’s crop varieties.

Editors and Affiliations

  • University of Adelaide, Australian Centre for Plant Functional Genomics (ACPFG), Urrbrae, Australia

    Delphine Fleury, Ryan Whitford

Bibliographic Information

  • Book Title: Crop Breeding

  • Book Subtitle: Methods and Protocols

  • Editors: Delphine Fleury, Ryan Whitford

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0446-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0445-7Published: 10 May 2014

  • Softcover ISBN: 978-1-4939-4324-1Published: 24 September 2016

  • eBook ISBN: 978-1-4939-0446-4Published: 09 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 255

  • Number of Illustrations: 9 b/w illustrations, 58 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access