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  • © 2014

Herpes Simplex Virus

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of the complex interactions between herpes viruses and the host
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1144)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. HSV-1 Biology and Life Cycle

    • Roger D. Everett
    Pages 1-17
  3. Herpes Simplex Virus Growth, Preparation, and Assay

    • Peggy Marconi, Roberto Manservigi
    Pages 19-29
  4. Engineering HSV-1 Vectors for Gene Therapy

    • William F. Goins, Shaohua Huang, Justus B. Cohen, Joseph C. Glorioso
    Pages 63-79
  5. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors

    • Matias E. Melendez, Cornel Fraefel, Alberto L. Epstein
    Pages 81-98
  6. HSV-1 Amplicon Vectors as Genetic Vaccines

    • Andrea S. Laimbacher, Cornel Fraefel
    Pages 99-115
  7. Modification of HSV-1 to an Oncolytic Virus

    • Hiroshi Nakashima, E. Antonio Chiocca
    Pages 117-127
  8. Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals

    • Andreas Sauerbrei, Kathrin Bohn
    Pages 149-165
  9. Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation

    • Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, Angus C. Wilson
    Pages 167-179
  10. Preparation of Herpes Simplex Virus-Infected Primary Neurons for Transmission Electron Microscopy

    • Monica Miranda-Saksena, Ross Boadle, Anthony L. Cunningham
    Pages 223-234
  11. Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

    • Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, Rebecca Vogel
    Pages 235-247
  12. Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components

    • Ryan H. Rochat, Corey W. Hecksel, Wah Chiu
    Pages 265-281
  13. In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells

    • Jolene M. Draper, Graham S. Stephenson, Jennifer H. LaVail
    Pages 283-292

About this book

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

Editors and Affiliations

  • Centre for Virus Research, Westmead Millennium Institute, Westmead, Australia

    Russell J. Diefenbach

  • Institute of Virology, University of Zürich, Switzerland

    Cornel Fraefel

Bibliographic Information

  • Book Title: Herpes Simplex Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Russell J. Diefenbach, Cornel Fraefel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0428-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0427-3Published: 27 March 2014

  • Softcover ISBN: 978-1-4939-5407-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0428-0Published: 26 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 331

  • Number of Illustrations: 17 b/w illustrations, 28 illustrations in colour

  • Topics: Infectious Diseases, Virology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access