Methods in Molecular Biology

Caspases,Paracaspases, and Metacaspases

Methods and Protocols

Editors: V. Bozhkov, Peter, Salvesen, Guy (Eds.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4939-0357-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA in USD
Softcover $129.00
price for USA in USD
About this book

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • General In Vitro Caspase Assay Procedures

    Pages 3-39

    Boucher, Dave (et al.)

  • Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity

    Pages 41-59

    Poręba, Marcin (et al.)

  • Global Identification of Caspase Substrates Using PROTOMAP (Protein Topography and Migration Analysis Platform)

    Pages 61-70

    Dix, Melissa M. (et al.)

  • Caspase-2 Protocols

    Pages 71-87

    Dorstyn, Loretta (et al.)

  • Caspase-14 Protocols

    Pages 89-100

    Yamamoto-Tanaka, Mami (et al.)

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4939-0357-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA in USD
Softcover $129.00
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Caspases,Paracaspases, and Metacaspases
Book Subtitle
Methods and Protocols
Editors
  • Peter V. Bozhkov
  • Guy Salvesen
Series Title
Methods in Molecular Biology
Series Volume
1133
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0357-3
DOI
10.1007/978-1-4939-0357-3
Hardcover ISBN
978-1-4939-0356-6
Softcover ISBN
978-1-4939-5405-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 266
Number of Illustrations
32 b/w illustrations, 34 illustrations in colour
Topics