Caspases,Paracaspases, and Metacaspases
Methods and Protocols
Editors: V. Bozhkov, Peter, Salvesen, Guy (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.
- Table of contents (16 chapters)
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General In Vitro Caspase Assay Procedures
Pages 3-39
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Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity
Pages 41-59
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Global Identification of Caspase Substrates Using PROTOMAP (Protein Topography and Migration Analysis Platform)
Pages 61-70
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Caspase-2 Protocols
Pages 71-87
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Caspase-14 Protocols
Pages 89-100
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Table of contents (16 chapters)
- Download Preface 1 PDF (35.3 KB)
- Download Sample pages 1 PDF (716 KB)
- Download Table of contents PDF (53 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Caspases,Paracaspases, and Metacaspases
- Book Subtitle
- Methods and Protocols
- Editors
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- Peter V. Bozhkov
- Guy Salvesen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1133
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-0357-3
- DOI
- 10.1007/978-1-4939-0357-3
- Hardcover ISBN
- 978-1-4939-0356-6
- Softcover ISBN
- 978-1-4939-5405-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 266
- Number of Illustrations
- 32 b/w illustrations, 34 illustrations in colour
- Topics
