Methods in Molecular Biology

Mitosis

Methods and Protocols

Editors: Sharp, David J. (Ed.)

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  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.

Table of contents (14 chapters)

Table of contents (14 chapters)
  • Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling

    Pages 3-30

    Brust-Mascher, Ingrid (et al.)

  • Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells

    Pages 31-40

    Decarreau, Justin (et al.)

  • Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Microcopy Images of C. elegans Embryos

    Pages 41-45

    Farhadifar, Reza (et al.)

  • Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells

    Pages 47-55

    Murata, Takashi (et al.)

  • Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics

    Pages 57-78

    Yang, Ge

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4939-0329-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $149.99
price for USA in USD
Softcover $129.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Mitosis
Book Subtitle
Methods and Protocols
Editors
  • David J. Sharp
Series Title
Methods in Molecular Biology
Series Volume
1136
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-4939-0329-0
DOI
10.1007/978-1-4939-0329-0
Hardcover ISBN
978-1-4939-0328-3
Softcover ISBN
978-1-4939-4301-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 308
Number of Illustrations
12 b/w illustrations, 71 illustrations in colour
Topics