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  • © 2014

Cerebral Angiogenesis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and key techniques to characterize cerebral angiogenesis
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1135)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xv
  2. Overview of Cerebral Angiogenesis

    1. Front Matter

      Pages 1-1
    2. Cerebral Angiogenesis During Development: Who Is Conducting the Orchestra?

      • Ina M. Wittko-Schneider, Fabian T. Schneider, Karl H. Plate
      Pages 3-20
    3. Vascular Normalization in Cerebral Angiogenesis: Friend or Foe?

      • Jisook Lee, Andrew Baird, Brian P. Eliceiri
      Pages 25-34
  3. Animal Models of Cerebral Angiogenesis

    1. Front Matter

      Pages 53-53
    2. Analysis of Angiogenesis in the Developing Mouse Central Nervous System

      • Nicole Ziegler, Karl H. Plate, Stefan Liebner
      Pages 55-68
    3. Hypoxia-Induced Angiogenesis and Capillary Density Determination

      • Constantinos P. Tsipis, Xiaoyan Sun, Kui Xu, Joseph C. LaManna
      Pages 69-80
    4. A Mouse Model of Chronic Cerebral Hypoperfusion Characterizing Features of Vascular Cognitive Impairment

      • Masafumi Ihara, Akihiko Taguchi, Takakuni Maki, Kazuo Washida, Hidekazu Tomimoto
      Pages 95-102
    5. A Mouse Model of Permanent Focal Ischemia: Distal Middle Cerebral Artery Occlusion

      • Kristian P. Doyle, Marion S. Buckwalter
      Pages 103-110
    6. A Method of Inducing Global Cerebral Ischemia

      • Gina Hadley, Michalis Papadakis, Alastair M. Buchan
      Pages 111-120
    7. Induction of Cerebral Arteriogenesis in Mice

      • André Duelsner, Nora Gatzke, Anja Bondke Persson, Ivo R. Buschmann
      Pages 121-125
    8. Vessel Painting Technique for Visualizing the Cerebral Vascular Architecture of the Mouse

      • Shea Hughes, Oleksandr Dashkin, Richard Anthony DeFazio
      Pages 127-138
    9. Examining Cerebral Angiogenesis in Response to Physical Exercise

      • Kiersten L. Berggren, Jacob J. M. Kay, Rodney A. Swain
      Pages 139-154
  4. Methods of Examining Cerebral Angiogenesis

    1. Front Matter

      Pages 155-155
    2. Histological Assessment of Angiogenesis in the Hypoxic Central Nervous System

      • Moises Freitas-Andrade, Jacqueline Slinn, Claudie Charlebois, Maria J. Moreno
      Pages 157-175
    3. Examining Vascular Remodeling in the Hypoxic Central Nervous System

      • Amin Boroujerdi, Jennifer V. Welser-Alves, Richard Milner
      Pages 177-186
    4. Analysis of Cerebral Angiogenesis in Human Glioblastomas

      • Michel Mittelbronn, Peter Baumgarten, Patrick N. Harter, Karl H. Plate
      Pages 187-203

About this book

Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process. Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.

Editors and Affiliations

  • Scripps Research Institute, La Jolla, USA

    Richard Milner

Bibliographic Information

  • Book Title: Cerebral Angiogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Richard Milner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0320-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0319-1Published: 09 February 2014

  • Softcover ISBN: 978-1-4939-4513-9Published: 27 August 2016

  • eBook ISBN: 978-1-4939-0320-7Published: 08 February 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 442

  • Number of Illustrations: 19 b/w illustrations, 83 illustrations in colour

  • Topics: Neurosciences, Neurobiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access