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Pulsed-Field Gel Electrophoresis

Protocols, Methods, and Theories

  • Book
  • © 1992

Overview

Editors:
  1. Margit Burmeister

    1. University of Michigan, Ann Arbor

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    You can also search for this editor in PubMed   Google Scholar

  2. Levy Ulanovsky

    1. Weizmann Institute of Science, Rehovot, Israel

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 12)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Electrophoretic Techniques

    1. Front Matter

      Pages 1-1
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    2. Field-Inversion Gel Electrophoresis

      • George F. Carle, Georges F. Carle
      Pages 3-18

    3. Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF)

      • Douglas Vollrath
      Pages 19-30

    4. Separation and Size Determination of DNA over a 10–,200 kbp Range

      • Craig A. Cooney
      Pages 31-37

    5. PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)

      • Margit Burmeister
      Pages 39-49

    6. Transverse Alternating-Field Electrophoresis

      • Katheleen Gardiner
      Pages 51-61

    7. Rotating Field Gel Electrophoresis (ROFE)

      • Andreas Ziegler, Armin Volz
      Pages 63-72

    8. Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE)

      • Jaan Noolandi, Chantal Turmel
      Pages 73-103

  3. Auxiliary Methods

    1. Front Matter

      Pages 105-105
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    2. Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis

      • Denise P.
      Pages 107-128

    3. Encapsulation of Cells in Agarose Beads

      • Joan Overhauser
      Pages 129-134

    4. Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE)

      • Jaan Noolandi, Chantal Turmel
      Pages 135-143

    5. Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis

      • Kun-Sheng Wu, Marion S. Riider, Martin W. Ganal
      Pages 145-157

    6. Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases

      • Bruno W. S. Sobral, Michael McCZeZZ
      Pages 159-172

    7. Desktop Digital Imaging

      • Gary A. Griess, Elena T. Moreno, Philip Sewer
      Pages 173-181

  4. Biological Applications

    1. Front Matter

      Pages 183-183
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    2. Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE)

      • Wilfried Bautsch
      Pages 185-201

    3. Protozoan Genomes

      • Lex H. T. Van der Ploeg, Keith M. Gottesdiener, Stanley H. Korman, Michael Weiden, Sylvie Le Blancq
      Pages 203-223

    4. Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis

      • Anthony P. Monaco, Zoia Lark, Hans Lehrach
      Pages 225-234
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Reviews

...an excellent reference and experimental manual...a must for every biochemistry and molecular biology library.-Journal of the American Chemical Society

Editors and Affiliations

  • University of Michigan, Ann Arbor

    Margit Burmeister

  • Weizmann Institute of Science, Rehovot, Israel

    Levy Ulanovsky

Bibliographic Information

  • Book Title: Pulsed-Field Gel Electrophoresis

  • Book Subtitle: Protocols, Methods, and Theories

  • Editors: Margit Burmeister, Levy Ulanovsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032299

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1992

  • Softcover ISBN: 978-1-4899-4391-0Published: 27 September 2013

  • eBook ISBN: 978-1-59259-499-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 481

  • Number of Illustrations: 99 b/w illustrations

  • Topics: Biochemistry, general

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