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Methods in Molecular Medicine

Hepatitis C Protocols

Editors: Lau, Johnson Y. N. (Ed.)

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About this book

In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as
animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed.

Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way
toward improved prevention, diagnosis, and treatment of viral hepatitis C.




Reviews

"Many leading researchers in the field are included in the book. . .It describes state-of-the-art methods and tools that are being used to address important medical and scientific questions. . .not only a highly practical manual for people working in laboratory medicine. . .also valuable for virologists, gastroenterologists, hepatologists and other medical infectious disease specialists."-Infection

"The useful clinical methods sections include description of methods for the detection of antibodies to HCV, quantitative HCV serum RNA assays, polymerase chain reaction (PCR) assays for the detection of HCV in liver and other tissues, HCV genotyping methods, and detection of HCV by in situ hybridization....Additional sections describe methods used by research, development, and production laboratories to clone and express HCV genes, to study viral replication and viral protein function, to produce recombinant HCV proteins, and to create in vitro culture models to allow study of viral replication and possible anitiviral compounds....The "Notes" sections will be particularly useful to individuals setting up the method for the first time. All the chapters contain current information and are well written....the scope of the book of use to anyone desiring to expand his or her knowledge of HCV. The introductory material for the sections is the best compilation of HCV-related information available in print today and makes Hepatitis C Protocols a valuable addition to the published literature on HCV."-Laboratory Medicine

"In summary Johnson Yiu-Nam Lau should be applauded for assembling an outstanding international ensemble of experts to produce a truly authoritative handbook for HCV clinicians and researchers."-The Quarterly Review of Biology

". . . an excellent review of the current and most up-to-date methods in molecular medicine involving hepatitis C. . . clinicians should review this textbook to give them a better understanding of the molecular techniques and shortcomings of the various diagnostic assays that they use in their day to day practice."-Canadian Journal of Infectious Disease

Table of contents (49 chapters)

  • Detection of Anti-GOR Antibodies in HCV Infection

    Manns, Michael P. (et al.)

    Pages 3-9

  • Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III

    Maertens, Geert (et al.)

    Pages 11-25

  • Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization

    Hodinka, Richard L.

    Pages 29-45

  • Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR)

    Qian, KePing

    Pages 47-53

  • The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA

    Gutekunst, Karen A. (et al.)

    Pages 55-69

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-1-59259-260-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $109.00
price for USA in USD
  • ISBN 978-1-4899-4348-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hepatitis C Protocols
Editors
  • Johnson Y. N. Lau
Series Title
Methods in Molecular Medicine
Series Volume
19
Copyright
1998
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-260-9
DOI
10.1385/0896035212
Softcover ISBN
978-1-4899-4348-4
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XXI, 621
Number of Illustrations and Tables
171 b/w illustrations
Topics