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Methods in Molecular Biology

Plant Cell Electroporation And Electrofusion Protocols

Editors: Nickoloff, Jac A. (Ed.)

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About this book

Gene transfer is an essential technology for improving our under­ standing of gene structure and function. Although there are many meth­ ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.

Table of contents (14 chapters)

Table of contents (14 chapters)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-542-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4899-4080-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Cell Electroporation And Electrofusion Protocols
Editors
  • Jac A. Nickoloff
Series Title
Methods in Molecular Biology
Series Volume
55
Copyright
1995
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-542-6
DOI
10.1385/0896033287
Softcover ISBN
978-1-4899-4080-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 206
Number of Illustrations
15 b/w illustrations
Topics