Methods in Molecular Biology

PCR Sequencing Protocols

Editors: Rapley, Ralph (Ed.)

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About this book

Advances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Reviews

...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. -FEBS Letters

"The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols....I highly recommend this most comprehensive volume for all molecular biologists."- SMG Quarterly

Table of contents (26 chapters)

  • Preparation and Analysis of DNA Sequencing Gels

    Theophilus, Bimal D. M.

    Pages 1-9

  • Purification of PCR Products from Agarose Gels for Direct Sequencing

    Brosius, Frank C., III (et al.)

    Pages 11-21

  • Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing

    Igloi, Gabor L.

    Pages 23-28

  • Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [α-35 S] dATP

    Casanova, Jean-Laurent

    Pages 29-34

  • Direct Sequencing by Thermal Asymmetric PCR

    Mazars, Georges-Raoul (et al.)

    Pages 35-40

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-551-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Spiral binding $139.99
price for USA in USD
  • ISBN 978-0-89603-344-3
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4899-4038-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
PCR Sequencing Protocols
Editors
  • Ralph Rapley
Series Title
Methods in Molecular Biology
Series Volume
65
Copyright
1996
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-551-8
DOI
10.1385/0896033449
Spiral binding ISBN
978-0-89603-344-3
Softcover ISBN
978-1-4899-4038-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 221
Number of Illustrations and Tables
24 b/w illustrations
Topics