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Protocols for Neural Cell Culture

Editors: Fedoroff, Sergey, Richardson, Arleen (Eds.)

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  • ISBN 978-1-4757-2586-5
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About this book

This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin­ ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul­ tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul­ tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo­ dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.

Reviews

The authors...lay strong laboratory background for a wide variety of studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience. Four Stars! -Doody's Health Sciences Book Review Journal

Table of contents (20 chapters)

  • Outgrowth Assays and Cortical Slice Cultures

    Castellani, Valérie (et al.)

    Pages 1-12

  • Microexplant Cultures of the Cerebellum

    Rogister, Bernard (et al.)

    Pages 13-23

  • Aggregating Neural Cell Cultures

    Honegger, Paul (et al.)

    Pages 25-49

  • Primary Cell Cultures of Peripheral and Central Neurons and Glia

    Johnson, Mary I. (et al.)

    Pages 51-75

  • Chick Spinal Somatic Motoneurons in Culture

    Juurlink, Bernhard H. J.

    Pages 77-89

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-1-4757-2586-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Protocols for Neural Cell Culture
Editors
  • Sergey Fedoroff
  • Arleen Richardson
Copyright
1997
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4757-2586-5
DOI
10.1007/978-1-4757-2586-5
Edition Number
2
Number of Pages
XX, 278
Number of Illustrations and Tables
27 b/w illustrations
Topics