Biological Separations

Methods of Protein Separation

Editors: Catsimpoolas, Nicholas (Ed.)

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About this book

This open-end treatise on methods concerning pro tein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles för achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaining insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection and/or modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of pro teins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area. Nicholas Catsimpoolas Cambridge, Massachusetts vü CONTENTS Contents of Volume 1 . xvii Chapter 1 Scanning Gel Cbromatography Gary K. Ackers I.

Table of contents (8 chapters)

  • Scanning Gel Chromatograpy

    Ackers, Gary K.

    Pages 1-26

  • Analytical and Preparative Polyacrylamide Gel Electrophoresis

    Chrambach, A. (et al.)

    Pages 27-144

  • Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis

    Rodbard, David

    Pages 145-179

  • Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis

    Rodbard, David

    Pages 181-218

  • Zone Electrophoresis, Isoelectric Focusing, and Displacement Electrophoresis (Isotachophoresis) in Carrier-Free Solution

    Hjertén, Stellan

    Pages 219-231

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-1-4684-9984-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $109.00
price for USA in USD
  • ISBN 978-1-4684-9986-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Methods of Protein Separation
Editors
  • Nicholas Catsimpoolas
Series Title
Biological Separations
Copyright
1976
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4684-9984-1
DOI
10.1007/978-1-4684-9984-1
Softcover ISBN
978-1-4684-9986-5
Edition Number
1
Number of Pages
XVIII, 326
Number of Illustrations and Tables
69 b/w illustrations
Topics