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Advances in Experimental Medicine and Biology

Immobilized Biochemicals and Affinity Chromatography

Editors: Dunlap, R. (Ed.)

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About this book

This volume contains most of the papers presented at the Sym­ posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi­ cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel­ lulose, glass, Sepharose, or polyacrylamide and exploits the princi­ ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish­ ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Affinity Chromatography — Old Problems and New Approaches

    Pages 3-14

    March, Steven C. (et al.)

  • Affinity Chromatography. New Approaches for the Preparation of Spacer Containing Derivatives and for Specific Isolation of Peptides

    Pages 15-31

    Wilchek, Meir

  • Quantitative Parameters in Affinity Chromatography

    Pages 33-42

    Nishikawa, A. H. (et al.)

  • Non-Specific Binding of Proteins by Substituted Agaroses

    Pages 43-59

    Hofstee, B. H. J.

  • A Solid Phase Radioimmune Assay for Ornithine Transcarbamylase

    Pages 61-73

    Eshenbaugh, Donald L. (et al.)

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-4684-6982-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $129.00
price for USA in USD
  • ISBN 978-1-4684-6984-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Immobilized Biochemicals and Affinity Chromatography
Editors
  • R. Dunlap
Series Title
Advances in Experimental Medicine and Biology
Series Volume
42
Copyright
1974
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4684-6982-0
DOI
10.1007/978-1-4684-6982-0
Softcover ISBN
978-1-4684-6984-4
Series ISSN
0065-2598
Edition Number
1
Number of Pages
XI, 377
Topics