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Emerging Biochemical and Biophysical Techniques

Modern Analytical Ultracentrifugation

Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems

Editors: Schuster, Todd M., Laue, Thomas M. (Eds.)

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About this book

There are numerous examples in the history of science when the parallel develop­ ments of two or more disciplines, methodologies, technologies or theoretical in­ sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu­ gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi­ mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer­ cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra­ centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Notes on the Derivation of Sedimentation Equilibrium Equations

    Pages 3-14

    Fujita, Hiroshi

  • Association of Rei Immunoglobulin Light Chain VL Domains: The Functional Linearity Of Parameters in Equilibrium Analytical Ultracentrifuge Models for Self-Associating Systems

    Pages 15-36

    Brooks, Ian (et al.)

  • Comments on the Analysis of Sedimentation Equilibrium Experiments

    Pages 37-65

    Johnson, Michael L. (et al.)

  • The Omega Analysis and the Characterization of Solute Self-Association by Sedimentation Equilibrium

    Pages 66-80

    Winzor, Donald J. (et al.)

  • Conservation of Signal: A New Algorithm for the Elimination of the Reference Concentration as an Independently Variable Parameter in the Analysis of Sedimentation Equilibrium

    Pages 81-93

    Minton, Allen P.

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-1-4684-6828-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.00
price for USA in USD
  • ISBN 978-1-4684-6830-4
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Modern Analytical Ultracentrifugation
Book Subtitle
Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems
Editors
  • Todd M. Schuster
  • Thomas M. Laue
Series Title
Emerging Biochemical and Biophysical Techniques
Copyright
1994
Publisher
Birkhäuser Basel
Copyright Holder
Birkhäuser Boston
eBook ISBN
978-1-4684-6828-1
DOI
10.1007/978-1-4684-6828-1
Softcover ISBN
978-1-4684-6830-4
Edition Number
1
Number of Pages
XIV, 354
Topics

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