MHC Molecules: Expression, Assembly and Function

Authors: Urban, Robert G., Chicz, Roman M.

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About this book

3 nant expression systems have been used to make MHC molecules con­ taining a single peptide of interest. To date, fifteen single peptide class I structures (incorporating three different HLA and two different H-2 allotypes/isotypes) and four additional class II structures (two single peptide complexes and two superantigen complexes) have been reported. These advances have enabled us to study the atomic detail of antigen presentation and the general mechanisms behind peptide binding, and begin to construct models of T cell recognition. Another area of research which has exploded over the past five years has been the identification of MHC-associated peptides. There are several methods one can use to determine the sequence identity of MHC restricted peptides. Historically, the most successful technique, albeit crude and encumbered with serious limitations, has been the use of overlapping synthetic peptides and T cell clones. Unfortunately, this method absolutely requires: (i) knowledge of the target antigen; (ii) availability of T cell clones; and (iii) a relatively short overall length for the target source protein, such that a set of overlapping pep tides can be affordably synthesized. Briefly, the entire sequence of the tar­ get protein is chemically synthesized using overlapping peptides which are then screened for biological activity using standard T cell presen­ tation assays. Despite its limitations, this method was used to identify the first immunodominant epitopes reported in the literature and con­ tinues to be used successfully today.

Table of contents (17 chapters)

  • Major Histocompatibility Antigens: An Introduction

    Chicz, Roman M. (et al.)

    Pages 1-8

  • The Major Histocompatibility Complex Genes and their Transcriptional Regulation

    Abdulkadir, Sarki A. (et al.)

    Pages 9-34

  • TAP Peptide Transporters and Antigen Presentation

    Momburg, Frank (et al.)

    Pages 35-63

  • Molecular Chaperones in MHC Class I and Class II Biosynthesis and Assembly

    Rajagopalan, Sumati

    Pages 65-71

  • Polypeptide Release from Lysosomes

    Isenman, Lois

    Pages 73-82

Buy this book

eBook $64.99
price for USA in USD (gross)
  • ISBN 978-1-4684-6462-7
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.00
price for USA in USD
  • Unpublished eBooks cannot be pre-ordered in advance.
  • Due: July 11, 1997
  • ISBN 978-1-4684-6464-1
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
MHC Molecules: Expression, Assembly and Function
Authors
Copyright
1996
Publisher
Springer US
Copyright Holder
R.G. Landes Company and Chapman & Hall
eBook ISBN
978-1-4684-6462-7
DOI
10.1007/978-1-4684-6462-7
Softcover ISBN
978-1-4684-6464-1
Edition Number
1
Number of Illustrations
52 b/w illustrations, 37 illustrations in colour
Topics