Separations Using Aqueous Phase Systems

1. Front Matter

   Pages i-xvii

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2. Partitioning of Macromolecules

   1. Front Matter

      Pages 1-1

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   2. General Aspects of Aqueous Polymer Two-Phase Partition

      • Per-Åke Albertsson

      Pages 3-5

   3. Affinity Partitioning of Enzymes

      • Göte Johansson

      Pages 7-14

   4. Dye Affinity Partitioning of Serum Proteins

      • Gerd Birkenmeier, Per-Ake Ålbertsson, Gerhard Kopperschläger

      Pages 15-23

   5. Hydrophobic Affinity Partitioning as a Method for Studying Conformational Changes in Proteins

      • V. P. Shanbhag, L. Backman

      Pages 25-31

   6. Affinity Partition of Enzymes with Dextran-Bound Procion Yellow HE-3G: Effect of Ligand Density

      • Monica Joelsson, Gōte Johansson

      Pages 33-34

   7. Rapid Two-Phase Extraction of the Calvin Cycle Enzymes Aldolase and Phosphoglycerate Kinase

      • Lars-Olof Persson, Björn Olde

      Pages 35-37

   8. Affinity Partitioning of Phosphofructokinase from Rat Erythrocytes in Poly(Ethylene Glycol)-Cibacron Blue/Dextran Two-Phase Systems

      • M. C. Tejedor, M. Grupelli, A. Rubio, C. Delgado, J. Luque

      Pages 39-40

   9. Studies on Triazine Dye-Enzyme Interaction by Means of Affinity Partitioning

      • Gerhard Kopperschläger, Jürgen Kirchberger, Thomas Kriegel

      Pages 41-42

3. Partitioning of Plant Membranes and Organelles

   1. Front Matter

      Pages 43-43

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   2. Separation of Organelles and Membranes by Phase Partitioning — A Short Overview

      • C. Larsson

      Pages 45-49

   3. Plasma Membrane Isolation by Phase Partition using a Single Partition Step: A Tool for the in vivo Study of Lipid Transfer to the Plasma Membrane

      • Patrick Moreau, Hélène Juguelin, René Lessire, Claude Cassagne

      Pages 51-58

   4. Free-Flow Electrophoresis Resolves Plasma Membrane Vesicles Isolated by Aqueous Two Phase Partition into Two Populations of Different Sidedness

      • Hervé Canut, Andrew Brightman, Alain Boudet, D. James Morré

      Pages 59-60

   5. Raising Monoclonal Antibodies Against Plasma Membranes from Barley Embryos

      • Ole Schou

      Pages 61-62

   6. Subfractionation of Cyanobacterial Thylakoids

      • Fredrik Nilsson, David J. Simpson, Alison C. Stewart, Bertil Andersson

      Pages 63-64

4. Partitioning of Animal Membranes and Organelles

   1. Front Matter

      Pages 65-65

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   2. Use of Phase Partitioning in Multidimensional Subcellular Fractionation

      • Austin K. Mircheff, Michael E. Bradley, Charles B. Hensley, Emile J. J. M. van Corven, Samuel C. Yiu, Ross W. Lambert

      Pages 67-80

   3. Comparison of Phase Partition with Alternative Methods for Isolation of Subcellular Structures

      • D. James Morré

      Pages 81-94

   4. Analysis of the Domain Structure of Membranes by Fragmentation and Separation

      • Per-Åke Albertsson

      Pages 95-99

The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

• Lipid
• biochemistry
• biology
• biophysics
• cell biology
• cell culture
• enzyme
• enzymes
• polysaccharide
• protein
• receptor

• Royal Free Hospital School of Medicine, London, UK

  Derek Fisher

• National Institute for Medical Research, London, UK

  Ian A. Sutherland

Policies and ethics