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Basic Life Sciences

Plant Transposable Elements

Editors: Nelson, O. (Ed.)

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About this book

Transposon tagging can work. Even though most of our understand­ ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in­ duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre­ gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de­ tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.

Table of contents (27 chapters)

  • Positive and Negative Regulation of the Suppressor-Mutator Element

    Fedoroff, N. (et al.)

    Pages 1-15

  • Germ Line and Somatic Mutator Activity: Are they Functionally Related?

    Robertson, Donald S. (et al.)

    Pages 17-42

  • The Mobile Element Systems in Maize

    Peterson, Peter A.

    Pages 43-68

  • Genetic and Molecular Analysis of Transposable Elements in Antirrhinum Majus

    Carpenter, Rosemary (et al.)

    Pages 69-80

  • Recombinant Mutable Alleles of the Maize R Gene

    Kermicle, Jerry L.

    Pages 81-89

Buy this book

eBook $99.00
price for USA in USD (gross)
  • ISBN 978-1-4684-5550-2
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $129.00
price for USA in USD
  • ISBN 978-1-4684-5552-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Transposable Elements
Editors
  • O. Nelson
Series Title
Basic Life Sciences
Series Volume
47
Copyright
1988
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4684-5550-2
DOI
10.1007/978-1-4684-5550-2
Softcover ISBN
978-1-4684-5552-6
Edition Number
1
Number of Pages
XI, 404
Topics