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Enzyme-Mediated Immunoassay

Editors: Lenhoff, H.M., Ngo, That T. (Eds.)

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About this book

T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Introduction

    Pages 1-2

    Ngo, T. T. (et al.)

  • Enzyme Mediated Immunoassay: An Overview

    Pages 3-32

    Ngo, T. T.

  • Separation-Free Enzyme Immunoassay for Haptens

    Pages 33-55

    Jaklitsch, Anna

  • Enzyme Modulator as Label in Separation-Free Immunoassays: Enzyme Modulator Mediated Immunoassay (EMMIA)

    Pages 57-72

    Ngo, T. T.

  • Prosthetic Group Labeled Enzyme Immunoassay

    Pages 73-84

    Ngo, T. T.

Buy this book

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  • ISBN 978-1-4684-5012-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
Softcover  
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Bibliographic Information

Bibliographic Information
Book Title
Enzyme-Mediated Immunoassay
Editors
  • H.M. Lenhoff
  • That T. Ngo
Copyright
1985
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4684-5012-5
DOI
10.1007/978-1-4684-5012-5
Softcover ISBN
978-1-4684-5014-9
Edition Number
1
Number of Pages
498
Topics