Human Hybridomas and Monoclonal Antibodies

Editors: Engleman, Edgar (Ed.)

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About this book

Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech­ nical problems, and the first reports of hybrids secreting human immu­ noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga­ tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.

Table of contents (24 chapters)

  • Human Hybridomas and Monoclonal Antibodies

    Shay, Jerry W.

    Pages 5-20

  • Fusion Partners for Production of Human Monoclonal Antibodies

    Kozbor, Danuta (et al.)

    Pages 21-36

  • Production of Human Monoclonal Antibodies Using Epstein—Barr Virus

    Crawford, Dorothy H.

    Pages 37-53

  • The Epstein—Barr Virus-Hybridoma Technique

    Roder, John C. (et al.)

    Pages 55-70

  • Strategies for Stable Human Monoclonal Antibody Production

    Reyes, Gregory R. (et al.)

    Pages 71-91

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4684-4949-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $159.99
price for USA in USD
  • ISBN 978-1-4684-4951-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Hybridomas and Monoclonal Antibodies
Editors
  • Edgar Engleman
Copyright
1985
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4684-4949-5
DOI
10.1007/978-1-4684-4949-5
Softcover ISBN
978-1-4684-4951-8
Edition Number
1
Number of Pages
XXIV, 526
Number of Illustrations and Tables
115 b/w illustrations
Topics