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Techniques in Somatic Cell Genetics

Authors: Shay, Jerry W.

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  • ISBN 978-1-4684-4271-7
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About this book

Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito­ chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu­ lating following the characterization of cytoplasmic mutations. The produc­ tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.

Table of contents (36 chapters)

Table of contents (36 chapters)
  • Selection of Purine and Pyrimidine Nucleoside Analog Resistance in Mammalian Cells

    Pages 1-9

    Morrow, John

  • Techniques for Using HAT Selection in Somatic Cell Genetics

    Pages 11-21

    Choy, W. N. (et al.)

  • Techniques for Decreasing the Toxicity of Polyethylene Glycol

    Pages 23-34

    Mercer, W. Edward (et al.)

  • The Use of Dimethyl Sulfoxide in Mammalian Cell Fusion

    Pages 35-45

    Norwood, Thomas H. (et al.)

  • The Selection of Heterokaryons and Cell Hybrids Using the Biochemical Inhibitors Iodoacetamide and Diethylpyrocarbonate

    Pages 47-65

    Wright, Woodring Erik

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4684-4271-7
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $159.99
price for USA in USD
  • ISBN 978-1-4684-4273-1
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Techniques in Somatic Cell Genetics
Authors
Copyright
1982
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4684-4271-7
DOI
10.1007/978-1-4684-4271-7
Softcover ISBN
978-1-4684-4273-1
Edition Number
1
Number of Pages
XXVI, 568
Number of Illustrations
95 b/w illustrations
Topics

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