Skip to main content
Book cover

Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Book
  • © 2014

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1121)

This is a preview of subscription content, log in via an institution to check access.

Access this book

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (30 protocols)

  1. Basic Concepts and Enhancement of DNA Transfer via Electroporation

  2. SiRNA Electrotransfer

  3. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues

Keywords

About this book

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Editors and Affiliations

  • The University of Texas M.D. Anderson Cancer Center, Houston, USA

    Shulin Li, Jeffry Cutrera

  • Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, USA

    Richard Heller

  • IPBS CNR, Toulouse, France

    Justin Teissie

Bibliographic Information

  • Book Title: Electroporation Protocols

  • Book Subtitle: Preclinical and Clinical Gene Medicine

  • Editors: Shulin Li, Jeffry Cutrera, Richard Heller, Justin Teissie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4614-9632-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4614-9631-1Published: 08 February 2014

  • Softcover ISBN: 978-1-4939-5492-6Published: 27 August 2016

  • eBook ISBN: 978-1-4614-9632-8Published: 08 February 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 349

  • Number of Illustrations: 20 b/w illustrations, 49 illustrations in colour

  • Topics: Human Genetics, Gene Expression

Publish with us