Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 1994

Handbook for Rhizobia

Methods in Legume-Rhizobium Technology

Authors:

  1. Padma Somasegaran

    1. University of Hawaii NifTAL Project, Paia, USA

    View author publications

    You can also search for this author in PubMed   Google Scholar

  2. Heinz J. Hoben

    1. University of Hawaii NifTAL Project, Paia, USA

    View author publications

    You can also search for this author in PubMed   Google Scholar

Sections

Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (38 chapters)

  1. Front Matter

    Pages i-xvi
    PDF

  2. General Microbiology of Rhizobia

    1. Front Matter

      Pages 1-6
      PDF

    2. Collecting Nodules and Isolating Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 7-23

    3. Observing the Infection Process

      • Padma Somasegaran, Heinz J. Hoben
      Pages 24-30

    6. Quantifying the Growth of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 47-57

    7. Counting Rhizobia by a Plant Infection Method

      • Padma Somasegaran, Heinz J. Hoben
      Pages 58-64

  3. Back Matter

    Pages 75-77
    PDF

  4. Identification of Rhizobia

    1. Front Matter

      Pages 79-88
      PDF

    2. Developing Antisera

      • Padma Somasegaran, Heinz J. Hoben
      Pages 89-93

    3. Somatic Agglutination Reactions with Pure Cultures of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 94-101

    4. Agglutinating Antigens from Root Nodules

      • Padma Somasegaran, Heinz J. Hoben
      Pages 102-106

    5. Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion

      • Padma Somasegaran, Heinz J. Hoben
      Pages 107-111

    6. Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion

      • Padma Somasegaran, Heinz J. Hoben
      Pages 112-119

    7. Producing and Applying Fluorescent Antibodies

      • Padma Somasegaran, Heinz J. Hoben
      Pages 120-130

    8. Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay

      • Padma Somasegaran, Heinz J. Hoben
      Pages 131-139

    9. Identifying Rhizobia by Immunoblot

      • Padma Somasegaran, Heinz J. Hoben
      Pages 140-148

    10. Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 149-152
Back to top

About this book

Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.
Back to top

Keywords

Back to top

Authors and Affiliations

  • University of Hawaii NifTAL Project, Paia, USA

    Padma Somasegaran, Heinz J. Hoben

Back to top

Bibliographic Information

  • Book Title: Handbook for Rhizobia

  • Book Subtitle: Methods in Legume-Rhizobium Technology

  • Authors: Padma Somasegaran, Heinz J. Hoben

  • DOI: https://doi.org/10.1007/978-1-4613-8375-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag New York, Inc. 1994

  • Softcover ISBN: 978-1-4613-8377-2Published: 17 November 2011

  • eBook ISBN: 978-1-4613-8375-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XVI, 450

  • Topics: Agriculture, Forestry, Plant Sciences, Cell Biology, Microbiology, Biotechnology

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation