Physicochemical Methods in the Study of Biomembranes

1. Front Matter

   Pages i-xxi

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2. Intracellular Lipid Distribution, Transport, and Sorting

   • Gerrit van Meer, Ida L. van Genderen

   Pages 1-24

3. Biophysical Characterization of Membrane and Cytoskeletal Proteins by Sedimentation Analysis

   • Michael B. Morris, Gregory B. Ralston

   Pages 25-82

4. Monomolecular Layers in the Study of Biomembranes

   • Rudy A. Demel

   Pages 83-120

5. Differential Scanning and Dynamic Calorimetric Studies of Cooperative Phase Transitions in Phospholipid Bilayer Membranes

   • Qiang Ye, Rodney L. Biltonen

   Pages 121-160

6. Ektacytometry of Red Cells

   • Robert M. Johnson

   Pages 161-203

7. Spin-Label ESR Study of Molecular Dynamics of Lipid/Protein Association in Membranes

   • László I. Horváth

   Pages 205-245

8. NMR Methods for Measuring Membrane Transport

   • Philip W. Kuchel, Kiaran Kirk, Glenn F. King

   Pages 247-327

9. Determination of Soluble and Membrane Protein Structure by Fourier Transform Infrared Spectroscopy

   • Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert

   Pages 329-362

10. Determination of Soluble and Membrane Protein Structure by Fourier Transform Infrared Spectroscopy

    • Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert

    Pages 363-403

11. Determination of Soluble and Membrane Protein Structure by Fourier Transform Infrared Spectroscopy

    • Erik Goormaghtigh, Véronique Cabiaux, Jean-Marie Ruysschaert

    Pages 405-450

12. X-Ray Diffraction on Biomembranes with Emphasis on Lipid Moiety

    • Peter Laggner

    Pages 451-491

13. Back Matter

    Pages 493-509

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In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi­ tionally been applied to studies of model systems of lipids of well-defined com­ position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans­ port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.

• Biomembran
• Lipid
• membrane
• membrane transport
• protein
• proteins
• spectroscopy

`Chapters are well written and informative and have extensive lists of references..the book is, in general, well produced.'
Journal of Magnetic Resonance

• University of Antwerp, Antwerp, Belgium

  Herwig J. Hilderson

• University of Sydney, Sydney, Australia

  Gregory B. Ralston

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