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Membrane Potential Imaging in the Nervous System

Methods and Applications

  • Book
  • © 2011

Overview

  • Outlines methods and procedures in imaging cellular activity in neurons using voltage-sensitive dyes 2.

  • Provides all the necessary material in the form of a practical manual 3.

  • Written by accomplished leaders in the area of voltage-imaging

  • Includes supplementary material: sn.pub/extras

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Table of contents (14 chapters)

Keywords

About this book

The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.

Reviews

“This is an excellent ‘how to’ and methods approach to single, multiple, and populational photon imaging using various dyes optically in what is now recognized as a cutting edge technique. … This is a very technical book for intermediate to advanced neuroscientists and would be useful for both the office and laboratory. … I highly recommend this book for all interested audiences.” (Joseph J. Grenier, Amazon.com, April, 2015)

Editors and Affiliations

  • Université Joseph Fourier, Unité Inserm 836, Grenoble Institute of Neuroscience, Grenoble, France

    Marco Canepari

  • Dept. Cellular &, Molecular Physiology, Yale University School of Medicine, New Haven, USA

    Dejan Zecevic

About the editors

Dejan Zecevic (b. Belgrade 1948) is a Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential fluctuation in dendritic spines and fine branches. Marco (b. Milan 1970) is first class INSERM researcher (CR1) working at the Grenoble Institute of Neuroscience. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity.

Bibliographic Information

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