Stock up on textbooks with 40% off + FREE shipping or choose from thousands of Protocols eBooks at just 9.99 each!

Principles and Technical Aspects of PCR Amplification

Authors: van Pelt-Verkuil, E., Belkum, Alex van, Hays, John P.

Free Preview
  • Gives a broad overview of available PCR techniques and variants
  • Focusing on the PCR technique, not restricted to a few limited applications associated with a minor discipline
  • Chapters outlining appropriate quality control procedures are included
  • Written by scientists with a broad knowledge of the field of PCR techniques and PCR-related clinical research
see more benefits

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4020-6241-4
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4020-6240-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-90-481-7579-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes.

Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits their appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation.

This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control and quality assurance, variants and adaptations of the standard PCR protocol, quantitative PCR and in situ PCR. The reader of this manual will be excellently informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice.

Table of contents (13 chapters)

Table of contents (13 chapters)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4020-6241-4
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4020-6240-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-90-481-7579-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Principles and Technical Aspects of PCR Amplification
Authors
Copyright
2008
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media B.V.
eBook ISBN
978-1-4020-6241-4
DOI
10.1007/978-1-4020-6241-4
Hardcover ISBN
978-1-4020-6240-7
Softcover ISBN
978-90-481-7579-6
Edition Number
1
Number of Pages
XII, 330
Topics