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Subcellular Biochemistry

Reviews and Protocols in DT40 Research

Subcellular Biochemistry

Editors: Buerstedde, Jean-Marie, Takeda, Shunichi (Eds.)

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eBook $229.00
price for USA in USD (gross)
  • ISBN 978-1-4020-4896-8
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $349.99
price for USA in USD
  • ISBN 978-1-4020-4895-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $299.99
price for USA in USD
  • ISBN 978-94-017-8264-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.

Table of contents (43 chapters)

  • DT40 gene disruptions: a how-to for the design and the construction of targeting vectors

    Arakawa, Hiroshi (et al.)

    Pages 1-9

  • Immunoglobulin gene conversion or hypermutation: that's the question

    Buerstedde, Jean-Marie (et al.)

    Pages 11-24

  • Genome resources for the DT40 community

    Caldwell, Randolph B. (et al.)

    Pages 25-37

  • Chromosome engineering in DT40 cells and mammalian centromere function

    Brown, William R. A. (et al.)

    Pages 39-48

  • Function of RECQ family helicase in genome stability

    Seki, Masayuki (et al.)

    Pages 49-73

Buy this book

eBook $229.00
price for USA in USD (gross)
  • ISBN 978-1-4020-4896-8
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $349.99
price for USA in USD
  • ISBN 978-1-4020-4895-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $299.99
price for USA in USD
  • ISBN 978-94-017-8264-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Reviews and Protocols in DT40 Research
Book Subtitle
Subcellular Biochemistry
Editors
  • Jean-Marie Buerstedde
  • Shunichi Takeda
Series Title
Subcellular Biochemistry
Series Volume
40
Copyright
2006
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media B.V.
eBook ISBN
978-1-4020-4896-8
DOI
10.1007/978-1-4020-4896-8
Hardcover ISBN
978-1-4020-4895-1
Softcover ISBN
978-94-017-8264-7
Series ISSN
0306-0225
Edition Number
1
Number of Pages
XII, 477
Topics